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The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. -- Wikipedia

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Several assumptions such as normality, linear relationship, and homoscedasticity are frequently required in parametric statistical analysis methods. Data collected from the clinical situation or experiments often violate
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